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| Product Name | Trypsin Solution, 0.1%, Sterile (EDTA-Free) |
| Catalog No. | RERE-0200 |
| Description | Sterile trypsin solution at 0.1 percent without EDTA, filter-sterilised and positioned between the mild 0.05 percent and the conventional 0.25 percent grades. The intermediate strength suits monolayers that detach readily but tolerate the mildest formulation poorly, and keeps proteolytic exposure low without extending incubation times excessively. Handle aseptically and avoid repeated freeze-thaw. Unopened aliquots keep for 12 months at -20°C. |
| Intended Use | Dissociation of adherent lines that need more activity than the mildest grade but less than the conventional strength |
| Principle / Technology | Intermediate trypsin concentration balances detachment speed against surface-protein exposure |
| Specificity | Sterile 0.1 percent trypsin, filter-sterilised; no EDTA; 12 month shelf life |
| Reaction Conditions / Protocol | Warm before use, rinse the monolayer with a balanced salt wash, add enough solution to cover and monitor detachment, then quench with serum-containing medium |
| Storage Conditions | -20°C |
| Package Specifications | 100 mL |
| Key Features | Intermediate 0.1 percent strength; EDTA-free; filter-sterilised; 12 month shelf life |
For research use only, not for clinical use.
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