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| Product Name | Trypsin Solution, 0.05%, Sterile (EDTA-Free) |
| Catalog No. | RERE-0199 |
| Description | Sterile trypsin solution at 0.05 percent without EDTA, filter-sterilised for dissociation workflows where a milder proteolytic challenge is preferred. The reduced strength relative to the 0.25 percent grade limits over-exposure of cell-surface proteins, which helps with fragile lines, tight passaging schedules and harvests destined for surface-marker analysis. Handle aseptically and avoid repeated freeze-thaw. Unopened aliquots keep for 12 months at -20°C. |
| Intended Use | Gentle dissociation of sensitive adherent lines and harvests where minimal surface-protein exposure matters |
| Principle / Technology | Lower trypsin concentration moderates proteolysis of adhesion and surface proteins while still releasing monolayers |
| Specificity | Sterile 0.05 percent trypsin, filter-sterilised; no EDTA; 12 month shelf life |
| Reaction Conditions / Protocol | Warm before use, rinse the monolayer with a balanced salt wash, add enough solution to cover and monitor detachment closely, then quench with serum-containing medium |
| Storage Conditions | -20°C |
| Package Specifications | 100 mL |
| Key Features | Mild 0.05 percent strength; EDTA-free; filter-sterilised; 12 month shelf life |
For research use only, not for clinical use.
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