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| Product Name | Trypsin-EDTA Solution, 0.25%/0.02%, with Phenol Red, Sterile |
| Catalog No. | RERE-0389 |
| Description | The 0.25% trypsin level marks the conventional strength for everyday detachment of robust adherent lines, and pairing it with 0.02% EDTA accelerates release by withdrawing the calcium and magnesium that stabilize adhesion complexes. Phenol red colors the solution so any pH drift is obvious before the reagent reaches the cells. Filter-sterilised and frozen for stability, it suits routine passage, harvest and subculture. |
| Intended Use | Standard dissociation of adherent cultures for passaging, expansion and harvesting. |
| Principle / Technology | Trypsin proteolysis with EDTA-supported cation withdrawal releases cells from the growth surface. |
| Specificity | 0.25% trypsin; 0.02% EDTA; with phenol red; filter-sterilised. |
| Reaction Conditions / Protocol | Add to the washed monolayer, incubate until cells detach, then inactivate with complete medium. |
| Storage Conditions | -20°C; 12 months. |
| Package Specifications | 100 mL, 500 mL, 10 × 100 mL |
| Key Features | Workhorse 0.25% strength; phenol red indicator; sterile with multiple pack sizes. |
For research use only, not for clinical use.
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