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| Product Name | Trypsin-EDTA Solution (0.25%), with Phenol Red |
| Catalog No. | RESK-0244 |
| Description | This product is a trypsin-EDTA solution formulated in Hanks' Balanced Salt Solution (HBSS), containing 2.5 g/L trypsin (1:250) and 0.2 g/L EDTA with phenol red as a pH indicator, and free of CaCl2, MgCl2·6H2O, and MgSO4·7H2O. It is supplied as a 1x premixed working solution and can be used directly for the dissociation of tissues and adherent cell monolayers. The product is sterile filtered and has been tested for mycoplasma. Reliable dissociation performance has been demonstrated on strongly adherent cell lines such as 293T and HUVEC cells. |
| Intended Use | Dissociation of tissues and adherent cell monolayers, cell disaggregation from adherent surfaces, and tissue dispersion during primary cell passaging in routine cell culture; suitable for most continuous, strongly adherent cell lines. |
| Principle / Technology | Trypsin is a serine protease commonly found in the digestive system of vertebrates and is secreted by the pancreas as the inactive precursor trypsinogen. It hydrolyzes peptide chains primarily at the carboxyl side of lysine or arginine residues (except when the adjacent residue is proline). During cell dissociation, trypsin degrades extracellular adhesion proteins, releasing adherent cells from the culture surface and dispersing cell aggregates. EDTA is a chemical chelator that sequesters calcium and magnesium ions, further promoting cell detachment and separation. |
| Sample Type | Adherent cultured cells and cell monolayers (e.g., 293T, HUVEC), and tissues |
| Performance Range / Specifications | Sterility: sterile filtered Chelator: EDTA (0.2 g/L) Indicator: phenol red Classification: animal-derived Appearance: liquid pH range: 7.2-8.0 Osmolality: 270-320 mOsm/kg Easy to use: supplied as a 1x premixed solution for direct dissociation of tissues and cell monolayers |
| Reaction Conditions / Protocol | Supplied as a 1x premixed working solution; apply directly to tissues or cell monolayers for dissociation. |
| Components / Formulation | 2.5 g/L trypsin (1:250) in Hanks' Balanced Salt Solution (HBSS) 0.2 g/L EDTA With phenol red (pH indicator) Free of CaCl2, MgCl2·6H2O, and MgSO4·7H2O Supplied as a 1x ready-to-use premixed working solution |
| Storage Conditions | Store at -25 to -15 °C. Aliquoting before freezing at -25 to -15 °C is recommended; avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years |
| Package Specifications | 100 mL |
| Product Form | Liquid, 1x ready-to-use premixed working solution |
| Quality Control | Sterile filtered; tested for mycoplasma contamination. |
| Concentration | 2.5 g/L trypsin (1:250), 0.25%; 0.2 g/L EDTA |
| Source / Origin | Porcine pancreas (animal-derived) |
| pH Range / Optimal pH | 7.2-8.0 |
| Shipping Conditions | Shipped with ice packs |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15 °C; digestive activity decreases over prolonged storage. Aliquot and freeze; avoid long-term storage at 4 °C. |
| Recommended Buffer System | Hanks' Balanced Salt Solution (HBSS) |
| Application Notes / Precautions | Color variation may occur: CO2 exchange during cold-chain transport can shift the solution pH from 7.2-8.0 (light red) to around 6.8 (light yellow); this pH-driven color change is a common phenomenon and the discolored solution still dissociates cells effectively and can be used with confidence. Because this formulation contains EDTA, the digestion solution should be removed after dissociation: EDTA is a chemical chelator that sequesters calcium and magnesium ions and cannot be neutralized by serum, so cells should be thoroughly washed after digestion. Aliquot before freezing and avoid repeated freeze-thaw cycles. |
| Batch-to-Batch Consistency | Osmolality controlled at 270-320 mOsm/kg; pH controlled at 7.2-8.0. |
| Recognition Site | Cleaves peptide bonds at the carboxyl-terminal side of lysine and arginine residues (except when the adjacent residue is proline). |
For research use only, not for clinical use.
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