- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Trypsin-EDTA Solution (0.25%), Phenol Red-Free |
| Catalog No. | RESK-0240 |
| Description | Trypsin is a serine protease commonly found in the digestive system of vertebrates; it is secreted by the pancreas as the inactive zymogen trypsinogen and hydrolyzes proteins, cleaving peptide chains mainly at the carboxyl side of lysine or arginine residues (except when either is immediately followed by proline). This product is a trypsin-EDTA solution formulated in Hanks' Balanced Salt Solution (HBSS), containing 2.5 g/L trypsin (1:250) and 0.2 g/L EDTA, without CaCl2, MgCl2.6H2O, or MgSO4.7H2O, and without phenol red. It is supplied as a 1x working solution and can be used directly for dissociation of tissues and monolayer cells. The product has been tested for mycoplasma. |
| Intended Use | For routine dissociation of tissues and monolayer (adherent) cells during cell culture and passaging. The phenol red-free formulation is better suited for flow cytometry (avoiding phenol red interference with fluorescence signals), hormone-related research (e.g., estrogen-sensitive experiments such as breast cancer or endometrial cell studies), and serum-free or special culture systems. For research use only. |
| Principle / Technology | Trypsin is a serine protease that hydrolyzes peptide bonds primarily at the carboxyl side of lysine and arginine residues, digesting cell-surface and extracellular matrix proteins to detach adherent cells. EDTA acts as a chelator of calcium and magnesium ions, weakening cell-cell and cell-substrate adhesion and enhancing dissociation efficiency. |
| Sample Type | Tissues and adherent (monolayer) cultured cells; suitable for most continuous, strongly adherent cell lines |
| Performance Range / Specifications | Appearance: liquid Sterility: sterile-filtered Chelator: EDTA Classification: animal-derived pH range: 7.2-8.0 Osmolality: 270-320 mOsm/kg Mycoplasma: tested |
| Specificity | Cleaves peptide bonds at the carboxyl side of lysine or arginine residues (except when immediately followed by proline). |
| Reaction Conditions / Protocol | Supplied as a 1x ready-to-use working solution; apply directly to tissues or monolayer cells for dissociation according to routine cell culture procedures. |
| Components / Formulation | 2.5 g/L trypsin (1:250) and 0.2 g/L EDTA in Hanks' Balanced Salt Solution (HBSS); without CaCl2, MgCl2.6H2O, and MgSO4.7H2O; phenol red-free; 1x working solution. |
| Storage Conditions | Store at -25 to -15 °C. Aliquoting before frozen storage at -25 to -15 °C is recommended. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years when stored at -25 to -15 °C. |
| Package Specifications | 100 mL |
| Product Form | Liquid (1x ready-to-use working solution) |
| Quality Control | Sterile-filtered; pH 7.2-8.0; osmolality 270-320 mOsm/kg; mycoplasma tested. |
| Concentration | 0.25% trypsin (2.5 g/L, 1:250) with 0.2 g/L EDTA |
| Source / Origin | Porcine (pig pancreas); animal-derived |
| pH Range / Optimal pH | pH 7.2-8.0 |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | 2 years at -25 to -15 °C. Aliquot before frozen storage and avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for most continuous, strongly adherent cell lines. Phenol red-free formulation is compatible with flow cytometry, hormone-related research, and serum-free or special culture systems. |
| Recommended Buffer System | Hanks' Balanced Salt Solution (HBSS), without calcium and magnesium. |
| Application Notes / Precautions | Aliquot before storage to avoid repeated freeze-thaw cycles. Phenol red-free formulation avoids interference with fluorescence signals in flow cytometry and is recommended for estrogen-sensitive studies and serum-free or special culture systems. |
| Recognition Site | Carboxyl side of lysine (K) and arginine (R) residues, except when immediately followed by proline. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
