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| Product Name | Trypsin-EDTA Solution, 0.05%/0.02%, with Phenol Red, Sterile |
| Catalog No. | RERE-0387 |
| Description | Cell dissociation hinges on trypsin clipping the adhesion proteins that pin cells to the vessel, while EDTA chelates the calcium and magnesium ions those proteins depend on; the two act in concert to release monolayers with minimal trauma. At 0.05% trypsin this preparation is tuned for gentle passaging of sensitive lines, and phenol red is included so the operator can monitor the pH of the working aliquot. The solution is filtered to sterility. |
| Intended Use | Gentle detachment of adherent cultured cells for passaging, harvesting and downstream assays. |
| Principle / Technology | Trypsin proteolysis combined with EDTA-mediated cation depletion loosens cell-surface adhesion contacts. |
| Specificity | 0.05% trypsin; 0.02% EDTA; with phenol red; filter-sterilised. |
| Reaction Conditions / Protocol | Cover the monolayer, incubate briefly until cells round up, then neutralise with complete medium. |
| Storage Conditions | -20°C; 12 months. |
| Package Specifications | 100 mL |
| Key Features | Low-strength formulation for delicate lines; phenol red indicator; sterile filtered. |
For research use only, not for clinical use.
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