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| Product Name | Trypsin-EDTA Solution, 0.05%/0.02%, Sterile (Phenol Red-Free) |
| Catalog No. | RERE-0388 |
| Description | Identical dissociation chemistry to the phenol red version but with the indicator left out, this 0.05% trypsin and 0.02% EDTA blend suits laboratories whose readouts are sensitive to colorants. Trypsin shears adhesion proteins and EDTA strips the divalent cations beneath them, releasing cells cleanly for counting, reseeding or analysis. Filtration renders the solution sterile. |
| Intended Use | Detachment of adherent cells in assays where an added dye would interfere with optical measurements. |
| Principle / Technology | Proteolytic cleavage plus cation chelation dismantles the adhesion apparatus holding cells to the substrate. |
| Specificity | 0.05% trypsin; 0.02% EDTA; without phenol red; filter-sterilised. |
| Reaction Conditions / Protocol | Incubate the monolayer with the solution until cells detach, then quench with serum-containing medium. |
| Storage Conditions | -20°C; 12 months. |
| Package Specifications | 100 mL |
| Key Features | Indicator-free formulation; gentle 0.05% strength; sterile filtered. |
For research use only, not for clinical use.
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