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| Product Name | Trypsin-Ammonium Bicarbonate Solution, 0.25%/0.2%, Sterile |
| Catalog No. | RERE-0391 |
| Description | This dissociation blend replaces EDTA with 0.2% ammonium bicarbonate, a volatile buffer that carries away dissolved carbon dioxide and leaves essentially no residue as it equilibrates. The 0.25% trypsin component performs the proteolytic work of severing adhesion contacts, while the bicarbonate medium supports the chemistry in a form that evaporates cleanly during subsequent handling. It is filtered to sterility and held frozen. |
| Intended Use | Cell dissociation in workflows that benefit from a volatile, residue-free buffering component. |
| Principle / Technology | Trypsin cleaves adhesive proteins while ammonium bicarbonate provides buffering that dissipates without deposit. |
| Specificity | 0.25% trypsin; 0.2% ammonium bicarbonate; filter-sterilised. |
| Reaction Conditions / Protocol | Treat the monolayer until cells detach, then neutralise and process according to the protocol. |
| Storage Conditions | -20°C; 12 months. |
| Package Specifications | 100 mL |
| Key Features | Volatile-buffer formulation; standard 0.25% trypsin strength; sterile filtered. |
For research use only, not for clinical use.
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