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| Product Name | TrypLE Express Recombinant Trypsin-Like Enzyme |
| Catalog No. | ATR-CCR-0026 |
| Description | A recombinant, animal-origin-free cell dissociation enzyme that provides an alternative to porcine trypsin for the detachment of adherent mammalian cells in culture. The enzyme is manufactured under controlled recombinant conditions, ensuring lot-to-lot consistency. |
| Intended Use | Gentle enzymatic dissociation of adherent mammalian cells from culture vessels for routine cell passaging, single-cell preparation, and cell harvesting in research and bioproduction applications. |
| Principle / Technology | A recombinant microbial trypsin-like protease specifically cleaves peptide bonds at the carboxyl side of lysine and arginine residues in extracellular matrix and cell adhesion proteins, causing controlled cell detachment without compromising cell surface marker integrity. |
| Detection Method | Dissociated cells are recovered by centrifugation, resuspended in complete growth medium, and plated at the desired density; cell viability is assessed by trypan blue exclusion |
| Sample Type | Adherent mammalian cell lines including HEK293, CHO, HeLa, Vero, MDCK, MRC-5, and many others cultured in standard tissue culture vessels |
| Performance Range / Specifications | Recommended working concentration: 1× (ready-to-use); dissociation time: 2–5 minutes at 37°C for most cell lines; dilution: 1× enzyme is diluted 1:10 upon addition of serum-containing growth medium |
| Sensitivity / LOD | Effective dissociation of >95% of adherent cells within 5 minutes for most cell lines |
| Specificity | Recombinant enzyme targets lysine and arginine residues; does not affect cell surface receptors or antigens at recommended exposure times, preserving epitopes for flow cytometry and cell sorting applications |
| Reaction Conditions / Protocol | Aspirate culture medium, rinse cell monolayer with PBS without Ca2+/Mg2+, add sufficient TrypLE Express to cover the monolayer (1–2 mL per T75 flask), incubate at 37°C for 2–5 minutes until cells round up and detach with gentle tapping, add growth medium to inactivate, centrifuge, and resuspend in fresh medium |
| Components / Formulation | 1× TrypLE Express solution in PBS with 1 mM EDTA, phenol red pH indicator; supplied sterile and ready to use |
| Storage Conditions | 2–8°C protected from light; can be stored at room temperature for up to 2 weeks during active use; do not freeze |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 100 mL (sufficient for approximately 50–100 standard cell passaging procedures in T75 flasks) |
| Product Form | Ready-to-use sterile liquid in PETG bottle |
| Quality Control | Each lot tested for sterility (USP), mycoplasma, pH, osmolality, and cell dissociation activity using a standardized CHO cell detachment assay; recombinant nature ensures consistent enzyme activity |
| Key Features | Animal-origin-free manufacturing eliminates risks associated with porcine-derived trypsin; the recombinant enzyme is highly stable at room temperature; gentler dissociation preserves cell surface markers for downstream applications; dilutable 1:1 with PBS for specialized protocols |
For research use only, not for clinical use.
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