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| Product Name | Truncated T4 RNA Ligase 2 KQ Double Mutant for Pre-Adenylated Adapter Ligation (200 U/uL) |
| Catalog No. | IVDRM-0387 |
| Description | This truncated form of T4 RNA Ligase 2 carries the R55K and K227Q double mutation, which preserves ligation efficiency while strongly suppressing non-specific RNA joining events such as RNA concatenation and self circularisation. The enzyme specifically attaches a 5 prime pre-adenylated DNA or RNA adapter to the 3 prime hydroxyl of an RNA molecule, so no ATP is needed in the reaction, only the pre-adenylated adapter. This property makes it the standard choice for ligating adapters onto small RNAs such as miRNA when building small RNA cDNA libraries. |
| Intended Use | Small RNA adapter ligation and cDNA library construction with 5 prime pre-adenylated DNA or RNA adapters |
| Principle / Technology | ATP independent ligation joining 5 prime pre-adenylated adapters to the RNA 3 prime hydroxyl |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 5 KU; 20 KU; 100 KU |
| Key Features | R55K and K227Q double mutation suppresses RNA concatenation and self circularisation; no ATP required in the ligation reaction |
| Purity | Greater than or equal to 95% |
| Concentration | 200 U/uL |
| Readouts / Downstream Assays | Small RNA adapter ligation efficiency in cDNA library construction |
For in vitro diagnostic use.
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