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| Product Name | Tris-Ammonium Chloride Red Blood Cell Lysis Buffer, Sterile, pH 7.2 |
| Catalog No. | RERE-0226 |
| Description | Sterile Tris-ammonium chloride lysis buffer at pH 7.2, supplied filter-sterilised for the Tris-NH4Cl approach to red blood cell removal. The Tris-buffered formulation holds pH steady through the lysis step, which supports reproducible recovery of leucocytes and other nucleated cells from blood and tissue suspensions ahead of flow cytometry, immunophenotyping and culture setup. Unopened bottles keep for 12 months at 2-8°C. |
| Intended Use | Red blood cell removal by the Tris-NH4Cl method with pH-stable lysis conditions |
| Principle / Technology | Tris buffering maintains pH 7.2 while ammonium chloride lyses erythrocytes selectively |
| Specificity | Sterile solution; pH 7.2; filter-sterilised; 12 month shelf life |
| Reaction Conditions / Protocol | Add to the cell suspension at the ratio and incubation the protocol specifies, monitor lysis and wash with excess buffer before downstream use |
| Storage Conditions | 2-8°C |
| Package Specifications | 100 mL | 500 mL |
| Key Features | Tris-buffered at pH 7.2; filter-sterilised; 12 month shelf life |
For research use only, not for clinical use.
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