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| Product Name | trans-Zeatin Riboside |
| Catalog No. | RESK-0593 |
| Description | Zeatin riboside is a highly active and widely used natural cytokinin (cytokinins, CKs). Functionally, it not only promotes cell division, stimulates stem/shoot proliferation, inhibits root formation, and prevents leaf senescence, but also activates gene expression and metabolic activity. Natural cytokinin levels are high when mature plants are in their maximal growth phase. Cytokinins are recognized by a group of histidine protein kinases (HKs) responsible for signal perception, and the signal is transmitted to the nucleus through a multi-step phosphorelay, thereby activating the expression of target genes. Zeatin riboside exists as cis and trans isomers, and the trans isomer is relatively more active. This product is high-purity trans-zeatin riboside with a content greater than 98% and is commonly used as a growth regulator in plant tissue culture. |
| Intended Use | Used as a growth regulator (cytokinin riboside) in plant tissue culture. |
| Principle / Technology | trans-Zeatin riboside is a highly active natural cytokinin. Cytokinins are recognized by histidine protein kinases (HKs) responsible for signal perception, and the signal is relayed to the nucleus through multi-step phosphotransfer, activating target gene expression. It promotes cell division and shoot proliferation, inhibits root formation, prevents leaf senescence, and activates gene expression and metabolic activity. |
| Detection Method | Purity determined by HPLC at 268 nm. |
| Performance Range / Specifications | Synonym: N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine CAS No.: 6025-53-2 Molecular formula: C15H21N5O5 Molecular weight: 351.36 Appearance: White to yellow powder Melting point: 180 °C Solubility: Slightly soluble in water; readily soluble in acetic acid (1 M) or alkaline solutions (e.g., 1 M KOH, 1 M NaOH) Maximum UV absorption (pH 7.0): 212 nm & 270 nm Purity: >98% (HPLC, 268 nm) |
| Reaction Conditions / Protocol | Upon receipt, centrifuge the vial at low speed. Fully dissolve the powder in 2-5 mL of 1 M NaOH (1 M acetic acid or 1 M KOH may also be used). Then add double-distilled water or ultrapure water with stirring to prepare a stock solution of 1 mg/mL or higher, ensuring thorough mixing. Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. Dilute the stock solution with culture medium to the required concentration; prepare working solutions fresh before use. |
| Storage Conditions | Store at -25 to -15 °C for long-term storage. |
| Package Specifications | 10 mg, 50 mg, 100 mg, 250 mg, 1000 mg |
| Product Form | White to yellow powder |
| Purity | >98% (HPLC, 268 nm) |
| Concentration | Stock solution: 1 mg/mL or higher; dilute with culture medium to the required working concentration. |
| Molecular Weight | 351.36 |
| Source / Origin | A highly active, widely used natural cytokinin; natural cytokinin levels are high when mature plants are in their maximal growth phase. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. Prepare working solutions fresh before use. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Dilute the stock solution with plant tissue culture medium to the required concentration. |
| Recommended Buffer System | Dissolve the powder in 1 M NaOH (1 M acetic acid or 1 M KOH may also be used), then dilute with double-distilled water or ultrapure water. |
| Application Notes / Precautions | Centrifuge the vial at low speed upon receipt before opening. Aliquot stock solutions and avoid repeated freeze-thaw cycles. Working solutions should be prepared fresh before use. |
For research use only, not for clinical use.
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