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| Product Name | Phosphatidylcholine Colorimetric Assay Kit |
| Catalog No. | BLTR-0022 |
| Description | This phosphatidylcholine colorimetric assay kit uses enzymatic colorimetric method to accurately quantify phosphatidylcholine. It has high sensitivity, good linearity, and is beneficial for cell membrane and lipid transport - related research |
| Test Target | Phosphatidylcholine |
| Methodology | Enzymatic colorimetric (phospholipase D, choline oxidase, peroxidase) |
| Measurement Range | 0.05-5 mmol/L |
| Sample Type | Serum, plasma, cell membranes, tissue homogenates |
| Assay Time | 15 min at 37°C |
| Wavelength | 500 nm |
| Linearity | 0.05-5 mmol/L |
| Precision (Intra-assay CV) | ≤2% |
| Precision (Inter-assay CV) | ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤400 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤15 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤800 mg/dL (no significant interference) |
| Reagent Composition | Phospholipase D, choline oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 50 mL; R2: 10 mL |
| Calibration Type | External calibration |
| Calibration Levels | 3 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of phosphatidylcholine in biological samples for research on cell membrane structure, lipid transport and signal transduction |
| Cross - Reactivity | No significant cross - reactivity with other phospholipids |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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