Tn5 Transposase, Mutant Fast Form (in vitro transposition and tagmentation)

Name: Tn5 Transposase, Mutant Fast Form (in vitro transposition and tagmentation)
Price: Contact Alta DiagnoTech for price USD
Availability: InStock

Cat.No: IVDRM-0327 Datasheet

Quantities:

- +

Add To Cart

Product Details Related Products

Product Name	Tn5 Transposase, Mutant Fast Form (in vitro transposition and tagmentation)
Catalog No.	IVDRM-0327
Description	A mutant form of wild-type Tn5 transposase that recognises the inside, outside and mosaic end sequences of the Tn5 transposon, with fragments carrying the mosaic end showing the highest transposition efficiency. The enzyme inserts Tn5 transposons into target DNA rapidly and near-randomly, supporting both in vitro transgenesis and tagmentation-based sequencing library construction where simultaneous fragmentation and adapter insertion shortens workflows.
Intended Use	In vitro transgenesis; tagmentation-based sequencing library construction; transposon insertion into target sequences.
Principle / Technology	The mutant transposase cuts and re-ligates DNA at Tn5 end sequences, inserting transposon modules randomly across the target.
Sample Type	Genomic DNA, chromatin
Performance Range / Specifications	Recognises IE, OE and ME end sequences; highest efficiency on ME-containing fragments.
Specificity	Engineered mutant optimised for fast, random insertion.
Reaction Conditions / Protocol	Combine enzyme with transposon-end adapters and target DNA under tagmentation conditions.
Components / Formulation	Mutant Tn5 transposase with storage buffer.
Storage Conditions	Component A at -85 to -65 °C; remaining components at -25 to -15 °C
Shelf Life	1 year
Package Specifications	10 U, 100 U
Product Form	Enzyme solution
Key Features	Fast random insertion; dual research and library-prep utility.
Activity / Unit Definition	Transposition activity defined in U
Source / Origin	Recombinant mutant Tn5 transposase
Readouts / Downstream Assays	Tagmented DNA for sequencing libraries

Cat.No	Product Name	Price
IEC-HMM-0238	≥500,000 U/mL PNGase F for Broad N-Glycan Release from Glycoproteins	Add To Cart
IVDRM-0432	Recombinant DNase I, E. coli Expressed, RNase-Free	Add To Cart
IVDRM-0415	EcoRI Rapid Restriction Endonuclease, High Concentration (100 U/uL)	Add To Cart
IEC-HMM-0208	15 U/µL Glycerol-Free 65°C Reverse Transcriptase for RT-LAMP	Add To Cart
IVDRM-0081	Fast Restriction Enzyme SphI (Recognition Site: GCATG/C)	Add To Cart
IEC-HMM-0147	>=10 U/mg Alpha-Glucosidase for 405 nm PNPG Amylase Assays	Add To Cart
IVDRM-0583	High Affinity Porcine RNase Inhibitor, Ultra-Low Residual (40 U/uL)	Add To Cart
IEC-HMM-0143	>=10 U/mg Adenosine Deaminase for 265 nm Adenosine Hydrolysis Assays	Add To Cart
IEC-HMM-0110	High-Sensitivity Warm-Start Bst III DNA Polymerase, Glycerol-Free	Add To Cart
IVDRM-0488	Thermostable RNase H (5 U/uL)	Add To Cart

For in vitro diagnostic use.