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| Product Name | Tko RNA Helicase from Thermococcus kodakaraensis (100 ug/mL) |
| Catalog No. | IVDRM-0579 |
| Description | Expressed from the TK0306 gene of the thermophilic archaeon Thermococcus kodakaraensis KOD1, Tko RNA Helicase is an ATP-dependent enzyme of the DEAD-box family that specifically unwinds single-stranded stem-loop RNA. It shows only weak activity on double stranded RNA and single stranded DNA and does not act on double stranded RNA substrates carrying 5 prime or 3 prime overhangs. With an optimal temperature of 50°C it works as a tool for melting RNA secondary structure at reduced temperatures, and it requires 1 to 2 mM ATP or dATP together with 2 mM magnesium as cofactor. |
| Intended Use | RNA secondary structure analysis, post-transcriptional regulation studies and improving reverse transcription past stem-loop RNA |
| Principle / Technology | ATP-dependent DEAD-box helicase activity that unwinds single-stranded stem-loop RNA |
| Sample Type | RNA substrates carrying stem-loop secondary structure |
| Specificity | Specific for single-stranded stem-loop RNA; weak on double stranded RNA and single stranded DNA; inactive on overhang-ended double stranded RNA |
| Reaction Conditions / Protocol | Include 1 to 2 mM ATP or dATP and 2 mM magnesium in the reaction and incubate at 50°C |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 5 ug; 20 ug |
| Key Features | Optimal activity at 50°C for controlled melting of RNA structure; supports reverse transcription yield |
| Concentration | 100 ug/mL |
| Source / Origin | Thermococcus kodakaraensis KOD1 |
| Readouts / Downstream Assays | Reverse transcription efficiency and RNA structure melting assays |
For in vitro diagnostic use.
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