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| Product Name | Tissue Digestion Solution |
| Catalog No. | RESK-0205 |
| Description | Tissue Digestion Solution (10x) gently and rapidly digests and dissociates biological tissue samples into cell suspensions or cell clusters, which can be used for subsequent organoid establishment and cell isolation culture. It is broadly applicable to the in vitro primary culture dissociation of solid tumors (such as colorectal, lung, breast, endometrial, and pancreatic cancers) and most normal tissue specimens. This product is supplied as a 10x concentrate and must be diluted before use. |
| Intended Use | For the gentle, rapid enzymatic dissociation of solid tumor and normal tissue specimens into cell suspensions or cell clusters for organoid construction and primary cell isolation culture. |
| Principle / Technology | Collagenase-based enzymatic digestion of the tissue extracellular matrix to release cells; trypsin-free formulation for gentle dissociation. |
| Sample Type | Solid tumor tissues (e.g., colorectal, lung, breast, endometrial, pancreatic cancer) and most normal tissue specimens. |
| Performance Range / Specifications | Broad sample compatibility: suitable for solid tumors and most normal tissue specimens Gentle and rapid digestion |
| Reaction Conditions / Protocol | Supplied as a 10x concentrate; dilute to working concentration before use. |
| Components / Formulation | The main component is collagenase; trypsin-free. Supplied as a 10x concentrate. |
| Storage Conditions | Store at -20 °C and avoid repeated freeze-thaw cycles. After dilution to working concentration: stable for 1 month at -20 °C; store at 2-8 °C and use within 48 h. |
| Shelf Life | 24 months at -20 °C (concentrate). After dilution: 1 month at -20 °C; use within 48 h at 2-8 °C. |
| Package Specifications | 10 mL 30 mL 100 mL |
| Product Form | 10x liquid concentrate |
| Expiration Date / Stability | 24 months at -20 °C for the concentrate; diluted working solution is stable for 1 month at -20 °C and should be used within 48 h when stored at 2-8 °C. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles. Dilute the 10x concentrate to working concentration before use; use the diluted solution at 2-8 °C within 48 h. |
For research use only, not for clinical use.
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