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| Product Name | Thermostable Reverse Transcriptase V, Glycerol-Free (600 U/uL) |
| Catalog No. | IVDRM-0520 |
| Description | A high concentration, glycerol-free format of the fifth generation reverse transcriptase engineered from M-MLV (H-) Reverse Transcriptase. The enzyme tolerates reaction temperatures up to 60°C, keeps 70% of its activity after 30 minutes at 58°C, and synthesises full-length cDNA up to 10 kb, which suits RNA templates with complex secondary structure and low copy target detection. Supplied without glycerol and stored at 2 to 8°C with a 6 month shelf life, it serves as an enzyme raw material for IVD molecular diagnostics and gene expression analysis by RT-qPCR. |
| Intended Use | Reverse transcription for IVD molecular diagnostics and gene expression analysis by RT-qPCR |
| Principle / Technology | RNA directed DNA polymerase activity with heat tolerance up to 60°C in a glycerol-free formulation |
| Sample Type | RNA templates for reverse transcription |
| Specificity | Tolerates up to 60°C; 70% activity retained after 30 minutes at 58°C; cDNA up to 10 kb |
| Reaction Conditions / Protocol | Run reverse transcription at elevated temperature up to 60°C in the assay workflow |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 6 months |
| Package Specifications | 3 KU; 12 KU; 120 KU; 300 KU; 3,000 KU |
| Key Features | High concentration glycerol-free format; heat stable across structured and low copy RNA templates |
| Concentration | 600 U/uL |
| Readouts / Downstream Assays | RT-qPCR amplification curves from RNA templates |
For in vitro diagnostic use.
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