- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Thermostable ATP-Dependent DNA Ligase (40 U/uL) |
| Catalog No. | IVDRM-0503 |
| Description | A high temperature DNA ligase that joins the 5 prime phosphate and 3 prime hydroxyl ends of two oligonucleotides hybridised in adjacent positions on the same complementary target strand. Ligation proceeds only when both oligonucleotides are perfectly paired with the target and leave no gap between them, so single base substitutions can be detected directly in ligase detection reaction and ligase chain reaction formats. Using ATP as cofactor, the enzyme remains active across a broad 45 to 70°C range, which supports ligation at elevated temperatures and mutation introduction by incorporation of phosphorylated oligonucleotides during PCR amplification. |
| Intended Use | Allele-specific gene detection by ligase chain and ligase detection reactions, high temperature ligation and PCR-based mutagenesis |
| Principle / Technology | ATP dependent, gap-free ligation of adjacent perfectly paired oligonucleotides between 45 and 70°C |
| Sample Type | Oligonucleotide probes hybridised to a complementary target DNA strand |
| Specificity | Ligates only perfectly paired adjacent oligonucleotides with no gap; active across 45 to 70°C |
| Reaction Conditions / Protocol | Incubate with paired probe oligonucleotides and target DNA at 45 to 70°C in an ATP containing buffer |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2000 U; 10000 U |
| Key Features | Broad 45 to 70°C activity range; ATP cofactor; single base discrimination by gap-free pairing requirement |
| Concentration | 40 U/uL |
| Readouts / Downstream Assays | Allele discrimination in ligase detection reaction assays |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
