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| Product Name | ThermoSalt RNA Ligase 2, Thermostable and Salt-Tolerant (70 uM) |
| Catalog No. | IVDRM-0580 |
| Description | An engineered double-stranded RNA ligase that efficiently joins RNA strands in both intermolecular and intramolecular reactions when ATP is present. Compared with conventional T4 RNA Ligase 2 it combines two unusual tolerances, keeping more than 90% of its ligation activity at 70°C and over 50% of activity in 300 mM NaCl, so it copes with complex samples and demanding reaction conditions. Supplied at 70 uM with at least 95% purity and a His tag, it runs for one hour between 25 and 80°C and is stopped by 2 mM or more EDTA. |
| Intended Use | RNA ligation in complex samples and under high temperature or high salt conditions |
| Principle / Technology | ATP-dependent catalysis of intermolecular and intramolecular RNA strand joining |
| Sample Type | RNA substrates in complex or high salt reaction systems |
| Reaction Conditions / Protocol | Incubate at 25 to 80°C for 1 hour and stop the reaction with 2 mM or more EDTA |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2000 pmol |
| Key Features | More than 90% activity at 70°C and more than 50% activity in 300 mM NaCl; His tag aids downstream handling |
| Purity | Greater than or equal to 95% |
| Concentration | 70 uM |
| Recommended Buffer System | Storage: Tris-HCl, KCl, ammonium sulfate, DTT, EDTA and 50% glycerol at pH 7.5; reaction: Tris-HCl, magnesium chloride, DTT and 400 uM ATP at pH 7.5 |
| Readouts / Downstream Assays | Ligation products resolved by denaturing gel electrophoresis |
For in vitro diagnostic use.
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