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| Product Name | Thermolabile dsDNase (20 U/uL) |
| Catalog No. | IVDRM-0481 |
| Description | A double stranded DNA selective endonuclease with almost no activity on single stranded nucleic acids, Thermolabile dsDNase hydrolyses the phosphodiester bonds of duplex DNA to yield oligonucleotides bearing 5 prime phosphate and 3 prime hydroxyl ends. The enzyme is heat labile and inactivates rapidly at 65°C, so it clears genomic DNA contamination ahead of reverse transcription without the extra EDTA step required by classical DNase I protocols, shortening the workflow and safeguarding RNA quantification. It is supplied at 20 U/uL with a purity of at least 99%, and 5 minutes at 37°C digests 1 microgram of genomic DNA. |
| Intended Use | Removal of genomic DNA contamination from RNA samples before reverse transcription |
| Principle / Technology | Selective endonucleolytic digestion of double stranded DNA with rapid heat inactivation at 65°C |
| Sample Type | RNA samples; genomic DNA contamination |
| Reaction Conditions / Protocol | Incubate at 37°C for 5 minutes to digest genomic DNA, then heat at 65°C for 10 minutes to inactivate |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 1000 U; 20000 U |
| Key Features | Negligible activity on single stranded nucleic acids; heat labile at 65°C |
| Purity | Greater than or equal to 99% |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Genomic DNA clearance verified by gel or qPCR |
For in vitro diagnostic use.
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