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| Product Name | Terminal Deoxynucleotidyl Transferase for Homopolymer Tailing (20 U/uL) |
| Catalog No. | IVDRM-0430 |
| Description | Terminal deoxynucleotidyl transferase is a template independent DNA polymerase that adds deoxynucleotides repetitively to the 3 prime hydroxyl ends of oligonucleotides and single or double stranded DNA, and reactions require a primer of at least three bases. On RNA templates performance depends strictly on the acceptor RNA 3 prime terminal structure and the nucleotide supplied, and activity on RNA is generally lower than on DNA. Common uses include homopolymer tailing, tailing reactions for 5-RACE rapid amplification of cDNA ends, molecular cloning support and detection of DNA break ends in apoptosis research. |
| Intended Use | Homopolymer tailing, 5-RACE tailing reactions and detection of DNA fragmentation in apoptosis studies |
| Principle / Technology | Template independent repetitive addition of deoxynucleotides to 3 prime hydroxyl ends |
| Sample Type | Oligonucleotides; single and double stranded DNA; cDNA synthesis and cloning workflows |
| Reaction Conditions / Protocol | Requires a primer of at least 3 bases; activity on RNA templates depends on 3 prime terminal structure |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 500 U; 2500 U; 180000 U |
| Quality Control | Free of nickase and exonuclease residue contamination |
| Key Features | Template independent tailing; bulk 180000 U pack for high throughput tailing workflows |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Tailing length and efficiency by gel or sequencing |
For in vitro diagnostic use.
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