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| Product Name | TelN Protelomerase for Covalently Closed Linear DNA Preparation (5 U/uL) |
| Catalog No. | IVDRM-0420 |
| Description | TelN protelomerase originates from bacteriophage N15, where it participates in generating the linear prophage genome. Unlike eukaryotic telomerase it is a pure protein without any RNA component and carries cut ligation activity: after cleaving double stranded DNA at the centre of the 56 bp palindromic telRL site it leaves covalently closed hairpin ends, with the flanking telR and telL arms forming a doggybone shaped molecule. A single enzymatic step therefore converts circular plasmid DNA into a linear covalently closed dumbbell molecule, the core reaction of enzymatic linear DNA synthesis. End closure integrity exceeds 90 percent as assessed by gel electrophoresis after T5 exonuclease treatment. |
| Intended Use | Converting circular plasmid DNA into linear covalently closed DNA for enzymatic linear DNA synthesis |
| Principle / Technology | Cut ligation activity creating covalently closed hairpin ends inside the 56 bp telRL palindrome |
| Performance Range / Specifications | Double stranded DNA end closure integrity above 90% |
| Specificity | Recognises telRL, a 56 bp palindromic sequence flanked by telR and telL, cutting within the central telO palindrome |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 250 U; 1000 U; 5000 U |
| Quality Control | End closure integrity assessed by gel electrophoresis after T5 exonuclease treatment; cut ligation efficiency verified on supercoiled plasmid |
| Key Features | One step enzymatic conversion to covalently closed linear DNA; pure protein without RNA component |
| Concentration | 5 U/uL |
| Source / Origin | Bacteriophage N15 |
| Readouts / Downstream Assays | Cut ligation efficiency assay; T5 exonuclease resistance gel assay |
For in vitro diagnostic use.
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