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| Product Name | TaqI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0476 |
| Description | Recognising 5 prime T^CGA 3 prime, this rapid recombinant endonuclease digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it operates in the shared rapid series buffer at an optimal 37°C Dam methylation overlapping the sequence can block cutting, and TthHB8I is an isoschizomer with the same specificity. The enzyme is not heat inactivatable, so after digestion remove it by phenol chloroform extraction or column purification. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime T^CGA 3 prime within 5 to 15 minutes at 37°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | Dam methylation overlapping the sequence can block cleavage; isoschizomer TthHB8I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Single shared buffer across the rapid series; requires physical removal rather than heat inactivation |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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