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| Product Name | Taq DNA Ligase for Ligase Chain Reaction and Allele-Specific Detection |
| Catalog No. | IVDRM-0500 |
| Description | A thermostable DNA ligase that joins the 5 prime phosphate and 3 prime hydroxyl ends of two oligonucleotides hybridised in adjacent positions on the same complementary target strand. Ligation occurs only when both oligonucleotides are perfectly paired with the target and leave no gap between them, which makes single base substitutions directly detectable through ligase chain reaction and ligase detection reaction formats. The enzyme uses NAD+ as cofactor and stays active across 45 to 65°C, and it also supports introduction of mutations through incorporation of phosphorylated oligonucleotides during primer extension amplification. |
| Intended Use | Allele-specific detection by ligase chain reaction and ligase detection reaction, single nucleotide variation analysis and mutation introduction |
| Principle / Technology | NAD-dependent ligation of adjacent perfectly paired oligonucleotides on a complementary target strand between 45 and 65°C |
| Sample Type | Oligonucleotide probes hybridised to a complementary target DNA strand |
| Specificity | Ligates only perfectly paired adjacent oligonucleotides with no gap, enabling single base discrimination |
| Reaction Conditions / Protocol | Incubate with paired probe oligonucleotides and target DNA at 45 to 65°C in an NAD+ containing buffer |
| Storage Conditions | -20°C, avoid repeated freeze thaw; store the B component buffer at -80°C for periods beyond 30 days |
| Shelf Life | 1 year |
| Package Specifications | 1000 U; 2000 U; 10000 U |
| Key Features | Thermostable across 45 to 65°C; gap-free perfect pairing requirement enables single base discrimination |
| Shipping Conditions | Ice pack shipping |
| Readouts / Downstream Assays | Allele discrimination in ligase detection reaction assays |
For in vitro diagnostic use.
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