Tagmentation DNA Library Preparation Kit for Illumina

Name: Tagmentation DNA Library Preparation Kit for Illumina
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Cat.No: NG-QCY-0013 Datasheet

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Product Details Related Products

Product Name	Tagmentation DNA Library Preparation Kit for Illumina
Catalog No.	NG-QCY-0013
Description	A transposase-based DNA library preparation kit that combines fragmentation and adapter incorporation in a simplified enzymatic workflow.
Intended Use	Whole-genome sequencing, single-cell amplification product library preparation and metagenomic sequencing.
Principle / Technology	An engineered transposase fragments DNA and introduces adapter sequences in a single reaction, followed by termination, enrichment and cleanup.
Detection Method	Downstream Illumina sequencing and library quality control.
Sample Type	DNA samples, including low-input DNA and single-cell amplification products.
Performance Range / Specifications	24 or 96 reactions; tagmentation at 55°C for 10 minutes; supports low-input library preparation with reduced adapter-dimer formation.
Specificity	Optimized transposase chemistry reduces GC bias, background interference and false-positive microbial signals.
Reaction Conditions / Protocol	Perform tagmentation, terminate the transposase reaction, amplify with platform-compatible index primers and purify the completed library.
Components / Formulation	Transposase-based tagmentation reagents, termination reagent and library-amplification components.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	24 reactions; 96 reactions.
Product Form	Multi-component tagmentation DNA library preparation kit.
Quality Control	Workflow time, GC bias, background signal, adapter-dimer formation and microbial detection performance are evaluated.
Key Features	Fast one-step tagmentation; simplified workflow; low GC bias; low false-positive background; low-input compatibility; reduced adapter dimers.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Designed for Illumina sequencing platforms.
Application Notes / Precautions	Complete the termination step before PCR because residual transposase can inhibit amplification.

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For research use only, not for clinical use.