Universal Enzymatic Fragmentation DNA Library Preparation Kit

Name: Universal Enzymatic Fragmentation DNA Library Preparation Kit
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Cat.No: NG-QCY-0012 Datasheet

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Product Name	Universal Enzymatic Fragmentation DNA Library Preparation Kit
Catalog No.	NG-QCY-0012
Description	A universal enzymatic fragmentation library kit that combines DNA fragmentation, end repair and dA-tailing in one reaction.
Intended Use	PCR or PCR-free whole-genome, whole-exome and targeted-capture library construction.
Principle / Technology	Enzymatic fragmentation, end repair and dA-tailing occur in one tube, followed directly by adapter ligation, library enrichment and size selection.
Detection Method	Downstream next-generation sequencing and library quality control.
Sample Type	DNA from diverse species and sample sources.
Performance Range / Specifications	Input range 1 ng–1 µg; 24 or 96 reactions; fragmentation at 37°C for 5–30 minutes generated library peaks of approximately 300–550 bp from 100 ng human genomic DNA.
Specificity	Optimized chemistry improves library uniformity and high-GC-region coverage while tolerating EDTA and elevated pH.
Reaction Conditions / Protocol	Adjust fragmentation time for the target insert size, then proceed directly to adapter ligation, cleanup, enrichment and size selection.
Components / Formulation	Enzymatic fragmentation/end-preparation mixture, ligation reagents and library-enrichment components.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	24 reactions; 96 reactions.
Product Form	Multi-component enzymatic-fragmentation DNA library kit.
Quality Control	Library yield, fragment distribution, species compatibility, impurity tolerance and coverage uniformity are evaluated.
Key Features	One-tube fragmentation and end preparation; broad species compatibility; EDTA and pH tolerance; improved high-GC coverage; 1 ng–1 µg input range.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Compatible with Illumina and MGI sequencing platforms.
Application Notes / Precautions	Fragmentation time should be optimized for DNA quality, input amount and desired insert size.

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For research use only, not for clinical use.