Unique Dual Index Primers for Illumina Tagmentation Libraries

Name: Unique Dual Index Primers for Illumina Tagmentation Libraries
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Cat.No: NG-QCY-0050 Datasheet

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Product Name	Unique Dual Index Primers for Illumina Tagmentation Libraries
Catalog No.	NG-QCY-0050
Description	Unique dual-index primer sets for tagmentation-based DNA or RNA library preparation. Independent i5 and i7 index combinations help reduce index hopping and index misassignment during multiplex sequencing.
Intended Use	Tagmentation-based DNA/RNA library preparation, ATAC-seq, CUT&Tag, high-throughput sequencing and automated library construction.
Principle / Technology	Dual-index PCR amplification adds two independent sample identifiers to each library.
Detection Method	High-throughput sequencing.
Sample Type	Tagmentation-prepared DNA or RNA libraries.
Performance Range / Specifications	Up to 384 unique dual-index combinations; cross-contamination rate ≤0.1%; library-yield variation among indexes within ±20%.
Specificity	Independent dual indexes improve sample assignment accuracy in multiplex sequencing.
Reaction Conditions / Protocol	Add the selected dual-index primer combination during library amplification according to the compatible tagmentation workflow.
Components / Formulation	Tube-format unique dual-index primers or four 96-well index-primer plates.
Storage Conditions	Store under the low-temperature conditions specified for sequencing oligonucleotide reagents.
Package Specifications	24 reactions; 96 reactions.
Product Form	Indexed primer set in tube or plate format.
Quality Control	Index combinations are functionally verified for library yield, uniformity and low cross-contamination.
Key Features	384 index combinations; single-addition workflow; low index crosstalk; suitable for automation and high-throughput sequencing.
Compatibility	Illumina sequencing workflows and compatible tagmentation-based library preparation systems.
Application Notes / Precautions	Use index combinations appropriate for the planned pooling design and sequencing instrument.
Batch-to-Batch Consistency	Index performance and cross-contamination are controlled during release testing.

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For research use only, not for clinical use.