Pathogen Multiplex PCR Mix for DNA and RNA

Name: Pathogen Multiplex PCR Mix for DNA and RNA
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Cat.No: NG-QCY-0024 Datasheet

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Product Details Related Products

Product Name	Pathogen Multiplex PCR Mix for DNA and RNA
Catalog No.	NG-QCY-0024
Description	A hot-start multiplex PCR mix designed for complex DNA pathogen panels and direct amplification of reverse-transcription products from RNA pathogens.
Intended Use	Pathogen detection, environmental microorganism identification, food-safety testing and targeted sequencing of DNA or RNA pathogens.
Principle / Technology	Hot-start polymerase control and optimized multiplex buffer chemistry support uniform high-order amplification. Reverse-transcription products can enter PCR directly without purification.
Detection Method	Endpoint PCR or downstream targeted next-generation sequencing.
Sample Type	DNA templates and reverse-transcription products from RNA templates.
Performance Range / Specifications	Supports 20–1,000-plex panels and 20–85% panel GC content; 24 or 96 reactions.
Specificity	Optimized hot-start chemistry supports high specificity and uniform coverage across complex primer pools.
Reaction Conditions / Protocol	Prepare RNA targets by reverse transcription when required, add the resulting product directly to the multiplex PCR mix with a validated primer pool, and run a panel-specific program.
Components / Formulation	Hot-start pathogen multiplex PCR mix for DNA and reverse-transcribed RNA target panels.
Storage Conditions	Store at −30 to −15°C. Do not store above room temperature.
Package Specifications	24 reactions; 96 reactions.
Product Form	Ready-to-use multiplex PCR mix.
Quality Control	Microbial background and multiplex amplification performance are controlled during release testing.
Key Features	DNA and RNA target compatibility; direct use of reverse-transcription products; up to 1,000-plex amplification; broad GC compatibility; low background.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Compatible with reverse-transcription products and targeted sequencing workflows.
Application Notes / Precautions	Validate reverse-transcription input volume, primer balance and thermal conditions for each assay panel.

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For research use only, not for clinical use.