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| Product Name | Viral RNA Multiplex Amplicon Library Preparation Kit |
| Catalog No. | NG-QCY-0026 |
| Description | A two-stage reverse-transcription and multiplex PCR library preparation system for targeted sequencing of viral RNA genomes. |
| Intended Use | Viral RNA genome analysis and targeted sequencing library preparation. |
| Principle / Technology | RNA is reverse transcribed, followed by two PCR stages that amplify panel targets and generate sequencing-ready indexed libraries. |
| Detection Method | Targeted next-generation sequencing. |
| Sample Type | Viral RNA samples with reported Cq values from approximately 16 to 38. |
| Performance Range / Specifications | Workflow as short as 5 hours with less than 1.5 hours of hands-on time; 24 or 96 reactions; reported custom-panel coverage of 99.7%. |
| Sensitivity / LOD | Reported target detection across samples with Cq values up to approximately 38. |
| Specificity | Multiplex primer-panel enrichment selectively amplifies defined viral genome regions. |
| Reaction Conditions / Protocol | Perform reverse transcription, complete the first multiplex amplification, add indexes in the second PCR, then purify, quantify and sequence the library. |
| Components / Formulation | Reverse-transcription reagents, multiplex PCR library reagents and indexing-stage reagents. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 24 reactions; 96 reactions. |
| Product Form | Multi-module viral RNA library preparation kit. |
| Key Features | Broad viral RNA input range; rapid workflow; low hands-on time; high target coverage; multiplex genome enrichment. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with Illumina sequencing platforms. |
| Application Notes / Precautions | Coverage depends on viral load, primer-panel design and sample quality; validate panel performance for each virus and specimen type. |
For research use only, not for clinical use.
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