Viral RNA Multiplex Amplicon Library Preparation Kit
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Viral RNA Multiplex Amplicon Library Preparation Kit

Cat.No: NG-QCY-0026 Datasheet

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Product Name Viral RNA Multiplex Amplicon Library Preparation Kit
Catalog No. NG-QCY-0026
Description A two-stage reverse-transcription and multiplex PCR library preparation system for targeted sequencing of viral RNA genomes.
Intended Use Viral RNA genome analysis and targeted sequencing library preparation.
Principle / Technology RNA is reverse transcribed, followed by two PCR stages that amplify panel targets and generate sequencing-ready indexed libraries.
Detection Method Targeted next-generation sequencing.
Sample Type Viral RNA samples with reported Cq values from approximately 16 to 38.
Performance Range / Specifications Workflow as short as 5 hours with less than 1.5 hours of hands-on time; 24 or 96 reactions; reported custom-panel coverage of 99.7%.
Sensitivity / LOD Reported target detection across samples with Cq values up to approximately 38.
Specificity Multiplex primer-panel enrichment selectively amplifies defined viral genome regions.
Reaction Conditions / Protocol Perform reverse transcription, complete the first multiplex amplification, add indexes in the second PCR, then purify, quantify and sequence the library.
Components / Formulation Reverse-transcription reagents, multiplex PCR library reagents and indexing-stage reagents.
Storage Conditions Store at −30 to −15°C.
Package Specifications 24 reactions; 96 reactions.
Product Form Multi-module viral RNA library preparation kit.
Key Features Broad viral RNA input range; rapid workflow; low hands-on time; high target coverage; multiplex genome enrichment.
Shipping Conditions Ship at or below 0°C.
Compatibility Compatible with Illumina sequencing platforms.
Application Notes / Precautions Coverage depends on viral load, primer-panel design and sample quality; validate panel performance for each virus and specimen type.

For research use only, not for clinical use.

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