Long-Range Pathogen Multiplex PCR Master Mix

Name: Long-Range Pathogen Multiplex PCR Master Mix
Price: Contact Alta DiagnoTech for price USD
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Cat.No: NG-QCY-0021 Datasheet

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Product Details Related Products

Product Name	Long-Range Pathogen Multiplex PCR Master Mix
Catalog No.	NG-QCY-0021
Description	A high-order multiplex PCR master mix developed for simultaneous amplification of pathogen targets, including long amplicons and panels with broad GC content.
Intended Use	Targeted pathogen amplification for molecular detection and sequencing panel development.
Principle / Technology	An optimized polymerase and buffer system supports uniform annealing and high-order multiplex amplification across complex primer pools.
Detection Method	Endpoint PCR, capillary electrophoresis or downstream targeted next-generation sequencing.
Sample Type	DNA templates and reverse-transcription products from RNA targets.
Performance Range / Specifications	Supports 1–1,000-plex panels, 30–80% panel GC content and amplicons from 100 bp to 5 kb; 24 or 96 reactions.
Specificity	Optimized multiplex chemistry supports high target specificity and balanced amplification across primer pairs.
Reaction Conditions / Protocol	Combine the master mix with a validated multiplex primer pool and sample template, then run a panel-specific thermal program. Reverse-transcription products may be used directly without purification.
Components / Formulation	Long-range multiplex PCR master mix and reaction reagents for assay-specific primer pools.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	24 reactions; 96 reactions.
Product Form	Ready-to-use multiplex PCR master mix.
Quality Control	Microbial background and multiplex amplification performance are controlled during release testing.
Key Features	Up to 1,000-plex amplification; amplicons up to 5 kb; broad GC compatibility; direct use of reverse-transcription products; low microbial background.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Compatible with DNA targets and RNA targets after reverse transcription.
Application Notes / Precautions	Optimize primer balance, cycle number and annealing/extension conditions for each panel.

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For research use only, not for clinical use.