T7 RNA Transcription Enzyme Mix, Low-dsRNA Format
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T7 RNA Transcription Enzyme Mix, Low-dsRNA Format

Cat.No: IVDRM-0359 Datasheet

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Product Name T7 RNA Transcription Enzyme Mix, Low-dsRNA Format
Catalog No. IVDRM-0359
Description This pre-assembled enzyme blend centres on an engineered T7 RNA polymerase whose mutations suppress dsRNA byproduct formation during transcription by a wide margin. Supplied with linear double-stranded DNA from plasmids or PCR products as template, the mix copies the sequence downstream of the T7 promoter into single-stranded RNA, and modified nucleotides added to the reaction yield biotinylated or dye-labelled transcripts. It is stored at -25 °C to -15 °C with a 12-month shelf life and is supplied in 20 uL to 1 mL fills.
Intended Use In vitro synthesis of ordinary or labelled RNA for mRNA development, RNA marking with biotin or fluorophores, and related transcription workflows.
Specificity Engineered T7 polymerase with markedly reduced dsRNA byproduct; accepts blunt or 5-prime overhanging linear dsDNA templates
Storage Conditions -25 °C to -15 °C
Shelf Life 12 months
Package Specifications 20 uL, 100 uL, 200 uL and 1 mL packs
Product Form Liquid
Model Type T7 transcription enzyme blend with engineered low-dsRNA polymerase

For in vitro diagnostic use.

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