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| Product Name | T7 RNA Polymerase for In Vitro Transcription (50 U/uL) |
| Catalog No. | IVDRM-0351 |
| Description | Recombinantly expressed in E. coli from the phage T7 gene, this RNA polymerase transcribes RNA from double-stranded DNA templates carrying the T7 promoter, where the guanine marked as the first position of the promoter motif becomes the first transcribed base. Linearised plasmids and PCR products with blunt or 5′ protruding ends both work as templates, and NTPs serve as substrates, making the enzyme a cornerstone of mRNA vaccine and drug research. |
| Intended Use | In vitro transcription of RNA for mRNA vaccine and drug development; synthesis of RNA from T7 promoter plasmid or PCR templates. |
| Specificity | Promoter motif 5′-TAATACGACTCACTATAG-3′ with the marked guanine as first transcribed base; accepts NTP substrates |
| Storage Conditions | -20 °C |
| Shelf Life | 12 months |
| Package Specifications | 5000 U, 25000 U and 50000 U packs |
| Concentration | 50 U/uL |
| Source / Origin | Phage T7 RNA polymerase, recombinant expression in E. coli |
| Model Type | DNA-dependent RNA polymerase, T7 phage type |
| Starting Material / Tissue Site | Double-stranded linearised plasmid DNA or PCR products carrying the T7 promoter |
For in vitro diagnostic use.
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