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| Product Name | T7 Exonuclease (10 U/uL) |
| Catalog No. | IVDRM-0494 |
| Description | Encoded by gene 6 of bacteriophage T7, this exonuclease acts on double stranded DNA and RNA/DNA hybrid duplexes, cutting in the 5 prime to 3 prime direction from 5 prime phosphorylated or 5 prime hydroxylated nucleotides to release oligonucleotides and mononucleotides. It initiates digestion both from 5 prime termini and from nicks or gaps in duplex DNA or RNA/DNA hybrids, but it does not degrade double or single stranded RNA. These features support seamless cloning, preparation of single stranded DNA templates, construction of random mutation libraries and digestion of uncircularised linear templates. The preparation is free of nickase and RNase residue. |
| Intended Use | Seamless cloning, single stranded DNA template preparation, random mutation library construction and linear template removal |
| Principle / Technology | 5 prime to 3 prime exonucleolytic digestion of double stranded DNA and RNA/DNA hybrids from termini, nicks and gaps |
| Sample Type | Double stranded DNA; RNA/DNA hybrid duplexes |
| Reaction Conditions / Protocol | Incubate with the DNA substrate to digest linear or nicked template in the 5 prime to 3 prime direction |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5000 U |
| Quality Control | Free of nickase and RNase residue |
| Key Features | Acts on nicks and gaps as well as termini; does not degrade RNA |
| Concentration | 10 U/uL |
| Source / Origin | Bacteriophage T7 gene 6 |
| Readouts / Downstream Assays | Agarose gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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