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| Product Name | T7 DNA Ligase, Cohesive-End Specific (3000 U/uL) |
| Catalog No. | IVDRM-0592 |
| Description | From bacteriophage T7, T7 DNA Ligase is an ATP-dependent double-stranded DNA ligase that catalyses formation of a phosphodiester bond between adjacent 5 prime phosphate and 3 prime hydroxyl groups. Compared with T4 and T3 DNA Ligase, T7 DNA Ligase efficiently joins only cohesive ends and nicks and cannot carry out blunt-end ligation, which makes it the enzyme of choice when blunt and cohesive ends coexist and only the cohesive ends should be joined. |
| Intended Use | Cohesive-end and nick ligation where blunt-end joining must be avoided |
| Principle / Technology | ATP-dependent phosphodiester bond formation between adjacent 5 prime phosphate and 3 prime hydroxyl groups |
| Sample Type | Double stranded DNA carrying cohesive ends or nicks |
| Specificity | Ligates cohesive ends and nicks only; no blunt-end ligation |
| Reaction Conditions / Protocol | Incubate with the ATP containing ligation buffer under the recommended conditions |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 KU; 750 KU |
| Key Features | Selective cohesive-end ligation in mixed-end DNA populations; ATP dependent |
| Concentration | 3000 U/uL |
| Source / Origin | Bacteriophage T7 |
| Readouts / Downstream Assays | Ligation products resolved by agarose gel electrophoresis |
For in vitro diagnostic use.
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