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| Product Name | T5 Exonuclease for Seamless Cloning and Gibson Assembly (10 U/uL) |
| Catalog No. | IVDRM-0479 |
| Description | A double stranded DNA specific exonuclease that also acts as a single stranded DNA endonuclease, T5 Exonuclease digests from the 5 prime termini of single or double stranded DNA and can initiate from gaps or nicks in linear or circular duplex DNA, yet it leaves supercoiled double stranded DNA intact. These properties make it useful for clearing incompletely joined products from circularised dsDNA, degrading denatured DNA carried over from alkaline lysis plasmid preparation, removing linear and nicked DNA from plasmid samples and supporting seamless cloning by Gibson assembly. The preparation carries no detectable nicking activity and supports positive clone rates up to 100%. |
| Intended Use | Seamless cloning and Gibson assembly, plus removal of linear, nicked and denatured DNA |
| Principle / Technology | Processive 5 prime to 3 prime exonuclease digestion from termini, gaps and nicks of double stranded DNA |
| Sample Type | Linear and nicked double stranded DNA; denatured DNA from plasmid preparation |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 3 years |
| Package Specifications | 1000 U; 5000 U |
| Quality Control | Free of nicking enzyme contamination; positive clone rate up to 100% |
| Key Features | Leaves supercoiled dsDNA intact; removes linear and nicked templates for cleaner assembly |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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