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| Product Name | T4 RNA Ligase 2 Truncated K227Q Mutant for Small RNA Adapter Ligation (200 U/uL) |
| Catalog No. | IVDRM-0343 |
| Description | An MBP-fusion truncated mutant of T4 RNA ligase 2, the K227Q substitution strongly reduces non-specific ligation between RNA molecules, suppressing circularisation and concatemer formation. The enzyme specifically joins a 5′ preadenylated DNA or RNA adapter to the 3′ hydroxyl of RNA without requiring ATP, which makes it the standard choice for ligating adapters onto small RNAs during cDNA library construction. |
| Intended Use | Ligation of 5′ preadenylated adapters to small RNA for cDNA library construction; small RNA sequencing workflows. |
| Specificity | Free of detectable exonuclease, nickase, RNase and phosphatase contamination; requires 5′ preadenylated adapters and no ATP |
| Storage Conditions | -25 °C to -15 °C |
| Shelf Life | 24 months |
| Package Specifications | 5 KU and 20 KU packs |
| Purity | ≥95% |
| Concentration | 200 U/uL |
| Source / Origin | MBP-fusion truncated K227Q mutant of T4 RNA ligase 2, recombinant |
| Model Type | Truncated RNA ligase mutant (K227Q) |
For in vitro diagnostic use.
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