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| Product Name | T4 RNA Ligase 2 for dsRNA Nick Ligation and Circular RNA Synthesis (10 U/uL) |
| Catalog No. | IVDRM-0372 |
| Description | An ATP dependent double stranded RNA ligase with both intermolecular and intramolecular RNA joining activity, T4 RNA Ligase 2 preferentially seals nicks in double stranded RNA rather than joining single stranded RNA ends, and it requires adjacent 5 prime phosphate and 3 prime hydroxyl groups for ligation. The enzyme can also join the 3 prime hydroxyl of RNA to the 5 prime phosphate of DNA within a double stranded context. Typical workflows include sealing dsRNA nicks, constructing RNA-DNA junctions and synthesis of circular RNA. The preparation is free of exonuclease, nickase and RNase contamination. |
| Intended Use | dsRNA nick ligation, RNA to DNA strand joining in double stranded structures and circular RNA synthesis |
| Principle / Technology | ATP dependent ligation of adjacent 5 prime phosphate and 3 prime hydroxyl ends in double stranded RNA substrates |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 1000 U; 5000 U |
| Quality Control | Free of exonuclease, nickase and RNase residues |
| Key Features | Markedly higher activity on dsRNA nicks than on single stranded RNA ends; high ligation efficiency on double stranded substrates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Ligation of dsRNA nicks and circRNA formation |
For in vitro diagnostic use.
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