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| Product Name | T4 RNA Ligase 1 for ssRNA Adapter Ligation and RNA Circularisation (10 U/uL) |
| Catalog No. | IVDRM-0401 |
| Description | An ATP dependent ligase that joins single stranded RNA, single stranded DNA or mononucleotides by forming phosphodiester bonds between 5 prime phosphate and 3 prime hydroxyl ends, T4 RNA Ligase 1 works most efficiently on RNA to RNA ligation, less efficiently between ssDNA and ssRNA, and only weakly between ssDNA molecules. Typical applications include intermolecular and intramolecular RNA ligation for circular RNA preparation, attachment of 5 prime adapters in miRNA library construction, and oligonucleotide joining to single stranded cDNA in 5-RACE workflows. The preparation is free of nickase, exonuclease and RNase contamination. |
| Intended Use | RNA to RNA ligation, miRNA library 5 prime adapter attachment, 5-RACE oligonucleotide joining and RNA circularisation |
| Principle / Technology | ATP dependent phosphodiester bond formation between 5 prime phosphate and 3 prime hydroxyl ends of single stranded substrates |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5000 U |
| Quality Control | Free of nickase, exonuclease and RNase residues |
| Key Features | Highest efficiency on ssRNA ligation; supports both intermolecular and intramolecular joining |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Adapter ligation efficiency in miRNA library construction |
For in vitro diagnostic use.
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