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| Product Name | T4 Phage β-Glucosyltransferase for 5-hmC Detection (10 U/uL) |
| Catalog No. | IVDRM-0338 |
| Description | This glycosyltransferase moves the glucose moiety of UDP-glucose onto 5-hydroxymethylcytosine residues in double-stranded DNA, forming β-glucosyl-5-hydroxymethylcytosine that resists cleavage by the MunI restriction enzyme. The reaction underlies glucosylation-based protection and analysis of 5-hmC and supports NGS methylation sequencing methods such as EM-seq, ACE-seq and TAB-seq. |
| Intended Use | 5-hmC detection and analysis; NGS methylation sequencing including EM-seq, ACE-seq and TAB-seq; protection of 5-hmC sites from MunI digestion. |
| Detection Method | 5-hydroxymethylcytosine (5-hmC) in double-stranded DNA |
| Storage Conditions | -25 °C to -15 °C |
| Shelf Life | 24 months |
| Concentration | 10 U/uL |
| Activity / Unit Definition | Transfers the glucose of UDP-glucose to 5-hmC residues in double-stranded DNA, generating β-glucosyl-5-hmC |
| Source / Origin | T4 bacteriophage |
| Model Type | Glycosyltransferase (β-glucosyltransferase) |
For in vitro diagnostic use.
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