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| Product Name | T4 Gene 32 Protein, gp32 (5 ug/uL) |
| Catalog No. | IVDRM-0514 |
| Description | The product of T4 phage gene 32 is a single-stranded DNA binding protein essential for T4 phage DNA replication and repair. It binds cooperatively to transiently formed single-stranded regions and stabilises them, playing a structural role in T4 DNA replication. Beyond its native function it is widely used to stabilise and label single-stranded DNA regions for observation of intracellular DNA structures by electron microscopy, and reported applications include promoting restriction endonuclease digestion, improving the yield and efficiency of reverse transcription in RT-PCR, enhancing T4 DNA polymerase activity and increasing PCR yield. |
| Intended Use | Stabilisation of single-stranded DNA in PCR, RT-PCR, restriction digestion, T4 DNA polymerase reactions and electron microscopy |
| Principle / Technology | Cooperative binding and stabilisation of transiently formed single-stranded DNA regions |
| Sample Type | Single-stranded DNA regions in enzymatic reactions and imaging preparations |
| Specificity | Single-stranded DNA specific binding protein of 33 kDa, essential for T4 phage DNA replication and repair |
| Reaction Conditions / Protocol | Add to the reaction or preparation mix; inactivate by heating at 65°C for 20 minutes after use |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 250 ug; 5 mg; 25 mg |
| Key Features | Raises PCR and RT-PCR yield; promotes restriction digestion and enhances T4 DNA polymerase activity |
| Concentration | 5 ug/uL |
| Shipping Conditions | Ice pack shipping |
| Recommended Buffer System | Formulated with 50% glycerol |
| Readouts / Downstream Assays | Reaction yield and gel or electron microscopy readout of stabilised DNA |
For in vitro diagnostic use.
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