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| Product Name | T4 Endonuclease V (T4 PDG) (1 U/uL) |
| Catalog No. | IVDRM-0493 |
| Description | T4 Endonuclease V, also known as T4 PDG or pyrimidine dimer glycosylase, is a bifunctional DNA glycosylase that combines DNA N-glycosylase and AP lyase activities. It recognises and binds cis-syn cyclobutane pyrimidine dimers formed by ultraviolet irradiation, cleaves the glycosidic bond on the 5 prime side of the dimer to release the dimer and leave an AP site, and then uses its endonuclease activity to cut the phosphodiester bond at the AP site, creating a single nucleotide gap in the DNA strand. This makes it useful for DNA repair and damage studies and for damage detection in comet assays. Supplied at 1 U/uL, the preparation is at least 95% pure by SDS-PAGE, carries no non specific DNase residue and holds host genomic DNA below 0.05 copies per U. |
| Intended Use | DNA repair and damage research, UV induced damage detection and comet assays |
| Principle / Technology | Bifunctional glycosylase and AP lyase activity that releases cis-syn cyclobutane pyrimidine dimers and creates a single nucleotide gap |
| Sample Type | UV irradiated DNA containing cis-syn cyclobutane pyrimidine dimers |
| Specificity | Targets cis-syn cyclobutane pyrimidine dimers; combines N-glycosylase and AP lyase activities |
| Reaction Conditions / Protocol | Incubate with UV damaged DNA to cleave at pyrimidine dimers, then resolve the resulting single nucleotide gaps |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 1000 U; 500 U |
| Key Features | Dual glycosylase and AP lyase activity; no non specific DNase residue; host genomic DNA below 0.05 copies per U |
| Purity | Greater than or equal to 95% (SDS-PAGE) |
| Concentration | 1 U/uL |
| Readouts / Downstream Assays | Comet assay readout and gap formation at dimer sites |
For in vitro diagnostic use.
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