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| Product Name | T4 DNA Polymerase (3 U/uL) with Ultralow Host DNA Residual |
| Catalog No. | IVDRM-0332 |
| Description | This T4 DNA polymerase catalyses 5′ to 3′ DNA synthesis on a primed template and carries strong 3′ to 5′ exonuclease activity without any 5′ to 3′ exonuclease activity. Compared with the standard formulation, this preparation carries an ultralow burden of residual host nucleic acid, which suits demanding background-sensitive settings such as pathogen microbiome testing. |
| Intended Use | Blunt-ending of protruding 5′ or 3′ DNA termini; synthesis of labelled DNA probes by replacement reaction; second-strand synthesis in site-directed mutagenesis; ligation-independent cloning; NGS library construction. |
| Specificity | Residual host genomic DNA below 3 copies per 30 U |
| Storage Conditions | -20 °C |
| Shelf Life | 12 months |
| Package Specifications | 150 U and 750 U packs |
| Concentration | 3 U/uL |
| Activity / Unit Definition | 5′→3′ DNA polymerase activity with 3′→5′ exonuclease activity; 5′→3′ exonuclease activity absent |
| Source / Origin | T4 bacteriophage |
| Shipping Conditions | Dry ice |
| Model Type | DNA-dependent DNA polymerase with proofreading exonuclease |
For in vitro diagnostic use.
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