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| Product Name | T4 DNA Ligase for RNA End Labelling and Oligonucleotide Circularisation (5 U/uL) |
| Catalog No. | IVDRM-0499 |
| Description | With ATP as coenzyme, this T4 DNA Ligase catalyses phosphodiester bond formation between adjacent 5 prime phosphate and 3 prime hydroxyl termini of double stranded DNA at blunt or cohesive ends, and it can also join RNA strands and single stranded DNA within duplex structures, although it does not ligate fully single stranded nucleic acids. These properties suit labelling of RNA 3 prime ends, circularisation of RNA and DNA oligonucleotides and cloning of cDNA. The preparation shows high ligation efficiency and is free of exonuclease and nickase residue. |
| Intended Use | RNA 3 prime end labelling, circularisation of RNA and DNA oligonucleotides and cDNA cloning |
| Principle / Technology | ATP dependent ligation of adjacent 5 prime phosphate and 3 prime hydroxyl ends in duplex DNA substrates |
| Sample Type | Blunt or cohesive ended double stranded DNA; RNA and DNA oligonucleotides |
| Reaction Conditions / Protocol | Incubate with the substrate in an ATP containing ligation buffer appropriate to the end type being joined |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 50000 U |
| Quality Control | Free of exonuclease and nickase residue; high ligation efficiency |
| Key Features | Joins blunt and cohesive ends as well as RNA within duplexes; free of exonuclease and nickase residue |
| Concentration | 5 U/uL |
| Shipping Conditions | Ice pack shipping |
| Readouts / Downstream Assays | Ligation of oligonucleotide substrates |
For in vitro diagnostic use.
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