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| Product Name | T3 DNA Ligase (3000 U/uL) |
| Catalog No. | IVDRM-0502 |
| Description | An ATP dependent double stranded DNA ligase from bacteriophage T3 that efficiently joins adjacent 5 prime phosphate and 3 prime hydroxyl ends in duplex DNA, with ligation of A/T overhangs more efficient than that of C/G ends. It ligates cohesive and blunt ended molecules, the latter improved by PEG 6000, and repairs nicks in double stranded DNA or in DNA-RNA hybrid duplexes where the complete strand is DNA and the nicked strand may present DNA-DNA, DNA-RNA, RNA-DNA or RNA-RNA junctions. These properties allow generation of DNA-RNA and RNA-DNA fusion nucleic acids and template-dependent RNA ligation into longer RNA fragments for antisense oligonucleotide synthesis. The enzyme also tolerates higher NaCl concentrations than T4 DNA Ligase. |
| Intended Use | Molecular cloning, adapter ligation, linear DNA circularisation, nick repair, DNA-RNA fusion nucleic acids and antisense oligonucleotide synthesis |
| Principle / Technology | ATP dependent ligation of adjacent 5 prime phosphate and 3 prime hydroxyl ends in double stranded DNA and DNA-RNA hybrid substrates |
| Sample Type | Cohesive or blunt ended dsDNA; nicked DNA and DNA-RNA hybrid duplexes |
| Specificity | Higher ligation efficiency on A/T overhangs than on C/G ends; higher NaCl tolerance than T4 DNA Ligase |
| Reaction Conditions / Protocol | Incubate with the ligation substrate in an ATP containing buffer; add PEG 6000 to raise blunt end ligation efficiency |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 KU; 750 KU |
| Quality Control | Free of exonuclease, nickase, non-specific nuclease and RNase residue |
| Key Features | Repairs nicks across DNA-DNA, DNA-RNA, RNA-DNA and RNA-RNA junctions; high salt tolerance |
| Purity | Greater than or equal to 99% protein purity |
| Concentration | 3000 U/uL |
| Readouts / Downstream Assays | Ligation and nick repair readout of substrate conversion |
For in vitro diagnostic use.
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