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| Product Name | SYBR Green I Nucleic Acid Gel Stain (10,000× in DMSO) |
| Catalog No. | IVDRM-0111 |
| Description | SYBR Green I Nucleic Acid Gel Stain (10,000× in DMSO) from Alta DiagnoTech is a highly sensitive fluorescent dye for double-stranded DNA, widely used in agarose and polyacrylamide gel electrophoresis. The dye has a maximum excitation wavelength of 497 nm, with secondary excitation peaks at ~290 nm and ~380 nm. When bound to DNA, SYBR Green I emits fluorescence centered at 520 nm, making it compatible with a wide range of gel imaging instruments. This product is supplied as a 10,000× concentrated staining solution in DMSO. |
| Intended Use | Highly sensitive fluorescent staining of dsDNA in agarose and polyacrylamide gel electrophoresis. |
| Principle / Technology | SYBR Green I is a fluorescent dye that binds double-stranded DNA and produces a strong fluorescent signal upon excitation, enabling sensitive detection of nucleic acids in gels. |
| Detection Method | Maximum excitation at 497 nm with secondary excitation peaks at ~290 nm and ~380 nm; DNA-bound fluorescence emission centered at 520 nm. Compatible with a variety of gel detection instruments. |
| Sample Type | Double-stranded DNA (dsDNA). |
| Performance Range / Specifications | Very high sensitivity for dsDNA detection in agarose and polyacrylamide gels. |
| Sensitivity / LOD | Very high sensitivity for dsDNA staining. |
| Specificity | Selective fluorescent staining of double-stranded DNA. |
| Reaction Conditions / Protocol | Supplied as a 10,000× concentrate in DMSO; dilute according to standard SYBR Green I gel staining procedures. |
| Components / Formulation | SYBR Green I dye supplied as a 10,000× solution in DMSO. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 100 µL; 500 µL; 5× 500 µL; 10× 500 µL |
| Product Form | Liquid; 10,000× solution in DMSO. |
| Concentration | 10,000× |
| Expiration Date / Stability | Stable for 1 year when stored at -25 to -15°C. |
| Compatibility | Compatible with a wide range of gel imaging instruments thanks to its 497 nm excitation maximum, secondary excitation peaks at ~290 nm and ~380 nm, and 520 nm emission. |
For research use only, not for clinical use.
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