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| Product Name | 96-Well Cell Culture Plate, TC-Treated, Sterile |
| Catalog No. | IGM-HMM-0047 |
| Description | Tissue culture-treated polystyrene 96-well plate with hydrophilic modified surface for optimal adherent cell attachment and growth. Sterilized by gamma irradiation. Flat bottom with lid and condensation rings to minimize evaporation and contamination risk. |
| Intended Use | Adherent mammalian cell culture for cytotoxicity assays, proliferation studies, transfection optimization, cell-based ELISA, and high-content screening in 96-well microplate format. |
| Principle / Technology | Vacuum gas plasma treatment introduces oxygen-containing functional groups (hydroxyl, carboxyl, carbonyl) to polystyrene surface, creating negatively charged hydrophilic surface at physiological pH. This modified surface facilitates adsorption of cell adhesion proteins from serum-containing media and promotes integrin-mediated cell attachment. |
| Detection Method | Cell attachment efficiency with reference cell line (e.g., HeLa, HEK293); sterility to USP <71>; surface contact angle measurement; endotoxin by LAL assay |
| Sample Type | Adherent mammalian cell lines, primary cells with extracellular matrix pre-coating, stem cells on feeder or matrix protein layers |
| Performance Range / Specifications | Cell attachment >90% within 2 hours for standard cell lines; well-to-well growth uniformity <10% CV; evaporation <2% over 7-day culture in humidified incubator |
| Sensitivity / LOD | Single-cell seeding and clonal expansion possible; supports cell densities from 100 to 50,000 cells per well |
| Specificity | Surface treatment validated with epithelial, fibroblast, endothelial, and tumor cell lines; optimal for serum-containing culture conditions |
| Reaction Conditions / Protocol | Seed cells in 100-200 uL culture medium; incubate at 37 C, 5% CO2, >95% humidity; monitor confluence and passage or assay at desired density. |
| Components / Formulation | TC-treated crystal polystyrene plate body, polystyrene lid with condensation rings, alphanumeric well marking, individually wrapped with medical-grade Tyvek and film |
| Storage Conditions | 15-30 C; store flat in original packaging to maintain sterility; protect from UV and direct sunlight |
| Shelf Life | 36 months from sterilization date |
| Package Specifications | 1 plate per individually sealed pouch; 50-100 plates per case; bulk pack 20 plates with 20 lids |
| Product Form | Flat-bottom 96-well plate with lid, tissue culture treated, sterile, individually wrapped |
| Quality Control | Surface contact angle 45-55 degrees (hydrophilic); cell attachment >90% in QC test; sterility per ISO 11137; endotoxin <0.01 EU/mL; cytotoxicity negative (USP <87>) |
| Key Features | TC-treated for optimal cell attachment; individually wrapped for aseptic handling; condensation-ring lid design; flat bottom for microscopic imaging; alphanumeric well identification |
| Purity | Sterile (SAL 10^-6); endotoxin <0.01 EU/mL; cytotoxicity tested negative (ISO 10993-5); non-pyrogenic |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Crystal-grade polystyrene; vacuum plasma treatment in ISO Class 7 cleanroom under ISO 13485 |
| pH Range / Optimal pH | Cell culture medium pH 7.0-7.7; surface charge stable across physiological pH range; avoid strong acids and organic solvents |
| Shipping Conditions | Ambient; plate stacks secured with interleaving to prevent scratching of optical surfaces |
| Expiration Date / Stability | 3 years from sterilization when stored in original sealed packaging; TC treatment surface stable throughout shelf life |
| Regulatory / Compliance | ISO 13485:2016; CE marked; ISO 10993-5 cytotoxicity; USP <87> biological reactivity; FDA Master File available |
| Compatibility | Compatible with all commercial cell culture media; suitable for use with inverted microscopes, plate readers, automated washers, and high-content imaging systems; 127.76 x 85.48 mm ANSI/SLAS standard footprint |
| Recommended Buffer System | Compatible with DMEM, RPMI 1640, MEM, F-12, and all standard cell culture media with 5-20% fetal bovine serum supplementation |
| Application Notes / Precautions | For serum-free or low-serum conditions, pre-coat with poly-D-lysine, collagen, or fibronectin; do not touch plate bottom for imaging applications; maintain lid on plate except during access in biosafety cabinet |
| Batch-to-Batch Consistency | Surface contact angle controlled +/- 5 degrees; cell attachment verified with reference HeLa cells at 2, 24, 48 hours; lot release includes sterility and endotoxin certification |
For research use only, not for clinical use.
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