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| Product Name | Cell Scraper, Sterile, 25 cm Handle |
| Catalog No. | IGM-HMM-0058 |
| Description | Sterile disposable cell scraper with 25 cm pivoting blade for efficient harvesting of adherent cells from tissue culture flasks and dishes. Flexible thermoplastic elastomer blade minimizes surface scratching while providing complete cell sheet removal. |
| Intended Use | Mechanical detachment of adherent mammalian cells from tissue culture vessel surfaces when enzymatic dissociation is undesirable or for harvesting cells for protein, RNA, or DNA extraction without trypsinization. |
| Principle / Technology | Flexible blade conforms to culture surface contour while pivoting mechanism maintains optimal scraping angle. Soft elastomer blade edge effectively lifts cell sheets without gouging the TC-treated surface. Extended handle length reaches into T-175 and T-225 flasks. |
| Detection Method | Cell removal efficiency measured by residual cell count post-scraping; surface scratching evaluated by microscopic inspection; sterility per ISO 11137; cytotoxicity per ISO 10993-5 |
| Sample Type | Adherent cell monolayers on TC-treated surfaces, primary cells, iPSC-derived cells, co-culture systems, cells grown on extracellular matrix coatings |
| Performance Range / Specifications | Blade width approximately 18 mm; handle length 25 cm; >95% cell removal efficiency from T-75 flask; blade spans approximately 1.5 flask widths per pass |
| Sensitivity / LOD | Blade non-cytotoxic by direct contact assay; endotoxin <0.1 EU/scraper; no cell viability reduction vs pipette scraping control |
| Specificity | Blade material does not absorb proteins or nucleic acids; no interference with downstream Western blot or qPCR analysis |
| Reaction Conditions / Protocol | Open sterile pouch; grip handle; position blade at flask edge; apply gentle pressure and sweep across surface to detach cell sheet; collect cells with pipette in appropriate buffer. |
| Components / Formulation | Polystyrene handle (25 cm), thermoplastic elastomer (TPE) pivoting blade, individually sealed in medical-grade peel pouch, gamma sterilized |
| Storage Conditions | 15-30 C; store flat to prevent handle deformation; protect from direct UV light |
| Shelf Life | 36 months from sterilization date |
| Package Specifications | 100 scrapers individually wrapped per case; 25 scrapers per dispensing box |
| Product Form | Sterile disposable cell scraper with 25 cm handle and pivoting blade, individually wrapped |
| Quality Control | Sterility per ISO 11137 batch release; blade pivot function check 100%; cytotoxicity per ISO 10993-5 per lot; bioburden pre-sterilization monitoring; visual inspection for blade defects |
| Key Features | Pivoting blade maintains optimal angle; flexible blade minimizes surface damage; 25 cm handle for all culture vessel sizes; individually wrapped sterile; non-cytotoxic; endotoxin-free |
| Purity | Sterile (SAL 10^-6); non-cytotoxic; endotoxin <0.1 EU/unit; DNase/RNase-free; pyrogen-free |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade polystyrene and TPE; manufactured in ISO Class 8 cleanroom under ISO 13485; gamma sterilized |
| pH Range / Optimal pH | Materials inert at pH 3-11; blade compatible with PBS, tris buffers, and RIPA lysis buffer for cell harvesting |
| Shipping Conditions | Ambient; scrapers individually wrapped and packed in partitioned inner boxes; avoid crushing that may deform blades |
| Expiration Date / Stability | 3 years from sterilization; blade flexibility maintained; sterility valid while individual packaging intact |
| Regulatory / Compliance | ISO 13485:2016; CE marked; ISO 10993-5 cytotoxicity tested; ISO 11137 sterilization; FDA registered facility |
| Compatibility | Compatible with all standard TC-treated culture vessels: T-25, T-75, T-175, T-225 flasks; 100 mm, 150 mm dishes; 6-well, 12-well, 24-well plates (blade width fits multiwell format) |
| Recommended Buffer System | Compatible with PBS, Hanks BSS, Tris buffer, RIPA lysis buffer, and general cell lysis buffers containing detergents; blade material compatible with common extraction buffer components |
| Application Notes / Precautions | Use gentle, consistent pressure to avoid culture surface damage; scrape in overlapping parallel passes for complete cell removal; for protein extraction, pre-chill PBS and work quickly on ice; one scraper may be reused within the same flask for multiple washes but must not be reused between different culture vessels |
| Batch-to-Batch Consistency | Blade durometer (Shore A) controlled within +/- 5 units; pivot joint torque measured per production lot; blade edge profile verified by optical comparator per cavity batch |
For research use only, not for clinical use.
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