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| Product Name | Agarose (Molecular Biology Grade) |
| Catalog No. | IVDRM-0113 |
| Description | Agarose is a purified linear galactan hydrocolloid obtained from agar or agar-bearing seaweed. Structurally, it is a linear polysaccharide built from alternating β-D-galactopyranose and 3,6-anhydro-α-L-galactopyranose units linked by (1→4) glycosidic bonds. As a gelling reagent, it is widely used for routine nucleic acid analysis by gel electrophoresis or blotting techniques such as Northern and Southern blotting, and it is also suitable for protein applications such as radial immunodiffusion (RID). Key quality parameters include sulfate content (a purity indicator, since sulfate is the predominant ionic group in agarose), gel strength (the force required to fracture the gel), gelling point (the temperature at which an agarose solution solidifies on cooling, which differs from the melting point due to hysteresis), and electroendosmosis (EEO), the electrically driven movement of liquid through the gel that can affect separation efficiency. |
| Intended Use | Routine analysis of nucleic acids by agarose gel electrophoresis; nucleic acid blotting applications such as Northern and Southern blotting; protein applications such as radial immunodiffusion (RID). |
| Principle / Technology | Agarose dissolved in buffer forms a porous gel matrix upon cooling. Nucleic acids migrate through the matrix under an electric field and are separated by size via molecular sieving. Key matrix parameters include gel strength, gelling point, sulfate content, and electroendosmosis (EEO); internal convection caused by EEO can interfere with separation efficiency, as biopolymers typically migrate toward the negative electrode. |
| Sample Type | DNA and RNA samples; also suitable for protein samples in radial immunodiffusion (RID) assays. |
| Performance Range / Specifications | Gel strength: ≥ 1200 g/cm² Sulfate content: controlled as a purity indicator Electroendosmosis (EEO): characterized for reliable separation Gelling point: distinct from melting point due to solution hysteresis High cost-performance ratio; good stability |
| Reaction Conditions / Protocol | Dissolve the desired amount of agarose in electrophoresis buffer by heating, pour into the gel tray, and allow to solidify. For a 1% gel, allow a minimum gelling time of 30 minutes to ensure a homogeneous gel and sharp bands. |
| Components / Formulation | Purified linear galactan polysaccharide extracted from agar or agar-bearing seaweed. |
| Storage Conditions | Store dry at room temperature. |
| Shelf Life | 5 years. |
| Package Specifications | 100 g; 500 g |
| Product Form | Powder |
| Quality Control | Gel strength is verified to be ≥ 1200 g/cm²; gel softness is strength-related and may vary slightly between batches. The product is sterile and free of DNase/RNase. A 1% gel requires a minimum gelling time of 30 minutes for uniform, well-resolved bands. |
| Purity | Sulfate content is monitored as the purity indicator, as sulfate is the major ionic group present in agarose. |
| Expiration Date / Stability | Stable for 5 years when stored dry at room temperature. |
| Compatibility | Suitable for nucleic acid gel electrophoresis, Northern and Southern blotting, and radial immunodiffusion (RID). |
| Application Notes / Precautions | Diffuse bands are usually caused by insufficient gelling time or a non-uniform gel; allow at least 30 minutes for a 1% gel to solidify. High-sieving agarose and 3:1 agarose offer approximately 2% size resolution (e.g., a 30 bp fragment can be resolved from fragments differing by about 0.6 bp). Low melting point agarose is not suitable for pulsed-field gel electrophoresis, which requires very low EEO and high gel strength. This agarose is sterile and nuclease-free; low melting point agarose is suitable for cell-based applications such as viral plaque assays. Gel firmness is related to gel strength; agarose is a natural agar extract, so strength varies slightly between batches. Gels with a strength of ≥ 1200 g/cm² meet specification. |
| Batch-to-Batch Consistency | As a natural agar extract, gel strength may vary slightly from batch to batch; all released lots meet the gel strength specification of ≥ 1200 g/cm². |
For research use only, not for clinical use.
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