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| Product Name | StuI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0468 |
| Description | Recognising 5 prime AGG^CCT 3 prime, this rapid recombinant endonuclease produces blunt ended fragments and rapidly cuts plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at 10 U/uL and performs in the common rapid series buffer at 37°C, with heat inactivation at 80°C for 20 minutes. Dcm and EcoBI methylation possibly overlapping the site may affect cleavage, and the isoschizomers include AatI, Eco147I, PceI and SseBI. |
| Intended Use | Rapid blunt ended digestion for DNA fragment preparation and cloning |
| Principle / Technology | Site specific blunt cleavage at 5 prime AGG^CCT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dcm and EcoBI methylation may affect cleavage; isoschizomers include AatI, Eco147I, PceI and SseBI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Blunt end output; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Gel profile of digested DNA |
For in vitro diagnostic use.
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