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| Product Name | SspDI (KasI) Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0534 |
| Description | A conventional restriction enzyme that recognises and cuts 5 prime G^GCGCC 3 prime, SspDI (KasI) differs from the rapid series enzymes in needing a longer digestion to cut DNA substrates completely, yet it still uses the shared rapid series reaction buffer, so double digestion with other enzymes of that series is straightforward. Heat inactivation is achieved at 80°C for 20 minutes, and one unit fully digests 1 microgram of pBR322 in one hour at 37°C in a 50 microlitre reaction. KasI, DinI, EgeI, EheI, Mly113I, NarI, PluTI and SfoI are recognised isoschizomers. |
| Intended Use | Restriction digestion for DNA fragment cloning and double digestion with rapid series enzymes |
| Principle / Technology | Palindromic site specific cleavage at 5 prime G^GCGCC 3 prime in the shared rapid buffer |
| Sample Type | Double stranded DNA containing GGCGCC sites |
| Specificity | Isoschizomers KasI, DinI, EgeI, EheI, Mly113I, NarI, PluTI and SfoI; longer digestion needed for complete cutting |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C until complete; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U |
| Key Features | Conventional kinetics with shared buffer compatibility for double digestion |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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